Phytochemical analysis, in-vitro antioxidant, anti-inflammatory and insect antifeeding activity of methanolic extract of Limnophila indica (L.) Druce

Present research reports the chemical composition, total phenolics, antioxidant and insect antifeeding activity of the methanolic extract of the Limnophila indica (L.) Druce. GC-MS analysis of the extract led to identification of fifty-two components constituting 74.3% of total extract composition mainly dominated by β-monolinolein (12.9%) and linoelaidic acid (11.6%). The extract possesses significant amount of total phenolics, flavonoids and ortho-dihydric phenols. Antioxidant activity assessment via NO radical scavenging, DPPH radical scavenging, metal chelating of Fe+2, reducing power activity reveals the plant possesses potential antioxidant property with respective IC50 values 42.48±1.87, 31.98±0.59, 59.02±0.73, 53.28±1.21 µg/ml. In-vitro anti-inflammatory activity screening of the extract revealed the extract acting as potentially active anti-inflammatory agent with IB50 value 43.98±2.66. Insect antifeeding activity monitored through no choice leaf dip method against Spilosoma obliqua resulting dose and time dependent antifeeding activity.