Acaricidal activity of clove bud oil against Dermatophagoides farinae (Acari: Pyroglyphidae).

Volatile oil from the clove bud was extracted by petroleum ether using Soxhlet Extractor.The acaricidal activity was examined using direct contact and vapour phase toxicity bioassays.In a filter paper contact toxicity bio-assay,at 2.5 h after treatment,clove bud oil at a dose of 12.20 μg/cm2 killed all dust mites.As judged by 24-h LD50 values,potent fumigant action was observed with clove bud oil(12.20 μg/cm2),showing an adequate acaricidal activity against indoor Dermatophagoides farinae.