Comparative Electrophoretic Study between Rabbit and Human Seminal Fluid

Summary:  Cellulose acetate electrophoresis of rabbit seminal fluid give, after amidoschwartz staining, 19 protein bands, all migration towards the anode. Nine of them are present in at least 80% of the samples. With the acid of specific detection techniques, it was possible to demonstrate the presence of zinc in four bands, acid phosphatase activity in two and glycoprotein in three of the bands. Serum compounds seem to be present in small quantities. Study of fluids squeezed out from prostate, paraprostate, seminal vesicle and epididymal tail show few specific bands in these secretions: four in the case of prostate, three for paraprostate and one for seminal vesicle. Comparisons between human and rabbit seminal fluid electrophoresis show no relationship between these two types of secretion. Zusammenfassung:  Celluloseacetat-Elektrophorese des Seminalplasmas von Kaninchen ergab nach Amidoschwartz-Farbung 19 Protein-Bande, die alle zur Anode wandern. 9 von diesen finden sich in 80% der Proben. Mit Hilfe spezieller Techniken war es moglich, das Vorkommen von Zink in 4 Banden zu zeigen, Phosphataseaktivitat in 2 und Glykoprotein in 3 Banden. Serumanteile scheinen in kleinen Mengen vorhanden zu sein. Untersuchungen von Sekret aus Prostata, Paraprostata, Vesiculae seminalis und Cauda epididymis zeigen einige spezifische Banden: 4 bei Prostata, 3 bei Paraprostata und 1 bei Vesiculae seminalis. Die vergleichende Elektrophorese-Untersuchung an Seminalplasma von Kaninchen und Mensch zeigte keine Ahnlichkeiten.