GLUTATHIONE CYCLE IN DIQUAT NEUROTOXICITY: ASSESSED BY INTRASTRIATAL PRE-TREATMENT WITH GLUTATHIONE REDUCTASE
2013
Diquat (DQ) neurotoxicity mechanisms are unknown, although, it's systemic
toxicity is mediated by free radical reactions. The role of glutathione cycle
was assessed by glutathione reductase (GR) applied in the pre-treatment of DQ
poisoning. Wistar rats were used and tested compounds were administered
intrastriatally (i.s.) in one single dose. Total glutathione (tGSH),
glutathione disulfide (GSSG) and glutathione peroxidase (GPx) were measured
in the vulnerable brain regions (VBRs) (striatum, hippocampus and cortex), at
30 minutes, 24 hours and 7 days post treatment. Results from the intact and
the sham operated groups were not statistically different. Rapid spatial
spreading of oxidative stress was confirmed in the examined VBRs. Mortality
(30-40%, within 24hrs) and signs of lethargy were observed in the DQ group.
Activity of GPx activity was elevated and GSSG/GSH was higher in the examined
VBRs during the experiment, compared to the controls. The i.s. pre-treatment
with GR achieved neuroprotective role against DQ induced neurotoxicity, based
on animal survival, absence of lethargy and decreased GPx activity and
GSSG/GSH in the examined VBRs during the experiment, compared to the DQ
group. Our results confirmed that oxidation of GSH was the reason for the
reduced antioxidative defense against DQ neurotoxicity. [Projekat
Ministarstva nauke Republike Srbije, br. III41018]
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
65
References
3
Citations
NaN
KQI