The association study of IL-2RA and CD40 gene polymorphism and delayed encephalopathy after acute carbon monoxide poisoning

Objective To study the association between interleukin-2 receptor 2 chain(IL-2RA) rs2104286, one of immume-factor-related genes, and leukocyte differentiation antigen 40 (CD40) rs6074022 gene polymorphism and delayed encephalopathy after acute carbon monoxide poisoning(DEACMP). Methods 109 DEACMP patients from Han population in Northern Henan Province were selected as the case group, and 115 patients of acute carbon monoxide poisoning without delayed encephalopathy (ACMP) as the control group; rs2104286 and rs6074022 genotypes were determined with PCR-restriction fragment length polymorphism (PCR-RFLP) and fluorescence quantitative PCR.The comparison was represented by χ2 test. Results ⑴In DEACMP group, the rs2104286 genotypes (T/T, T/C, C/C) and alleles (T and C) were 92, 17, 0 and 201, 17, respectively.In contrast to 93, 22, 0 and 208, 22 in ACMP group, no significant difference was noticed (all P>0.05), and the rs6074022 genotypes (C/C, C/T, T/T) and alleles (C and T) were 17, 50, 42 and 84, 134, respectively.In contrast to 15, 52, 48 and 82, 148 in ACMP group, no significant difference was noticed (all P>0.05). ⑵ With regards to gender stratification, the rs2104286 and rs6074022 genotypes and alleles of female patients in both groups were not significantly different (all P>0.05); the rs2104286 and rs6074022 genotypes and alleles of male patients in both groups were not significantly different (all P>0.05). ⑶ No interactions between different genotypes of rs2104286 and rs6074022 and DEACMP occurrence were observed (all P>0.05), which indicated that the above interactions might not impact on the genetic susceptibility of DEACMP. Conclusion No evidence has yet shown the association between rs2104286 and rs6074022 gene polymorphism with DEACMP; IL-2RA and CD40 genes as genetic susceptibility genes of DEACMP are not temporarily supported. Key words: Carbon monoxide poisoning; Delayed encephalopathy; Interleukin-2 receptor α chain; Leukocyte differentiation antigen 40