Resposta imunológica a antígenos de Hysterothylacium deardorffoverstreetorum de peixes teleósteos
2017
Anisaquidosis is a disease caused by parasites of Anisakidae family and is characterized by
gastrointestinal and allergic reactions. The Anisakis simplex is a more pathogenic Anisakidae to humans
and is highly allergenic. However, other species of this family also have characteristics that are harmful
to humans, but little is known about the immunogenicity this parasites. The objective of this study was to
experimentally assess the immunogenic potential of the parasite Hysterothylacium
deardorffoverestreetorum (H.D) in mice. Mice of inbred BALB/c strain were divided into three groups
and received three immunizations of the following antigenic preparations obtained from L3 larvae H.D:
Crude larval extract of H.D (CEH) Extract secreted / excreted larvae H.D. (ESH) and crude extract of
larvae after excretion / secretion (EEH). Serum samples were obtained on different days after
immunization to determine the levels of circulating specific antibodies by enzyme-linked immunosorbent
assay (ELISA). The results show increased production of immunoglobulin (Ig) G after the second
immunization with a gradual increase after the third immunization. Regarding IgE reactivity, this
occurred later, demonstrating a progressive increase only after the third immunization. Cellular
immunity was evaluated by intradermal, and showed statistically significant result compared to the
control used. This experiment is the first description of the pathogenic potential of this parasite in
mammals and represents a breakthrough in the diagnosis of human Anisakidosis.
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