[Image analysis of cardiac muscle cytoskeleton under simulated microgravity based on gray-level co-occurrence matrix (GLCM)].

Objective To study morphological changes of the cytoskeleton-microtubule (MT) of the fetal rat cardiac myocytes under simulated microgravity, and to quantify its image by utilizing the gray level co-occurrence matrix (GLCM) parameters of the image. Method Cytoskeleton images, including cellular microphotographs taken under normal or microgravity(clinostat) conditions, were quantified by gray level co-occurrence matrix parameters,and the pharmacological counter effect of quercetin against the influences of microgravity was estimated with these parameters. Result The results showed that the texture of microtubules in the image became worse under simulated microgravity environment. It also showed that quercetin has certain counter effect against the influence of microgravity. Conclusion The microtubule of the cardiac myocytes cytoskeleton becomes diffused under microgravity, and the GLCM parameters can well describe these variation. Quercetin has certain counter-effect against the influence of microgravity.