Effect of SRC-1 deficiency on the expression and nuclear translocation of NF-κB in mice after burn injury

Objective To study the effect of SRC-1 deficiency on the expression and nuclear translocation of NF-κB in mice after burn injury.Methods SRC-1 knock out mice and wild mice were divided into expriment group and control group according to their genotype.The difference of expression of NF-κB in total hepatic protein extract was investigated before burn,1h after burn,6h after burn and 12h after burn.The difference of translocation of NF-κB into nuclear was also measured in hepatic nuclear eatract.At the same time,the serum samples were acquired before burn,1h after burn as well as 6h after burn.The levels of cytokines TNF-α,IL-1β as well as IL-6 in serum samples were measured.Results The level of NF-κB expression in total hepatic protein extract increased in SRC-1 knock-out mice,and declined in wild mice after burn injury.the nuclear translocation of NF-κB were all increased in two groups after burn,but the quantity of NF-κB in nuclear extract was more higher in SRC-1 knock out mice than that in wild mice.The level of serum TNF-α,IL-1β as well as IL-6 all increased in two groups.But much higher level of proinflammtory cytokine were observered in SRC-1 knock-out mice as compared with wild mice.Conclusion SRC-1 protein can protect body from burn stress,The abscent of SRC-1 enhanced the function of NF-κB after burn injury.As a result,more pro-inflammatory cytokines were scrected during burn injury and more severe symptoms were observed in SRC-1 knock-out mice after burn injury.