Method and arrangement for light microscopic examination of a sample sheet

The invention relates to a light sheet microscope which an illumination device, which produces coherent illumination light for multiple illumination wavelengths, a beam forming module (8) for generating a light sheet of illumination light, an illumination lens (10) for illuminating a sample (1) with the light sheet, and a detection lens (12 ), comprises for imaging light emitted from the sample to a planar detector, wherein the optical axis of the detection lens (12) with the optical axis of the illumination lens (10) includes a 0 ° and 180 ° angle different. are arranged in such a light sheet microscope, the beam forming module (8) comprises a phase-selective element (19) on which a plurality of spatially separate from each other, selection areas (20), wherein in each case a selection area (20) is associated with a specific illumination wavelength, and wherein each of the selection area ( 20) a predetermined for the respective illumination wavelength phase distribution is impressed. Also, the beam forming module (8) includes means for sequential or simultaneous selection of the selection areas (20) in dependence on the respective illumination wavelength.