Primer and kit for detecting channel catfish viruses through pyrosequencing technology and detecting method

The invention discloses a primer and kit for detecting channel catfish viruses through the pyrosequencing technology and a detecting method, and particularly relates to the primer for detecting channel catfish viruses through the pyrosequencing technology. The primer includes an amplification primer pair shown in SEQ ID No.2 and SEQ ID No.3 in the sequence list and a sequencing primer shown in SEQ ID No.4 in the sequence list. The invention further discloses the detecting kit with the primer. When channel catfish viruses are detected through the pyrosequencing technology, the advantages of high flux, rapidness and accuracy are achieved, short DNA sequence analysis can be accurately conducted, the time consumed by the sequencing process is short, and the standard operation procedure can be conveniently established.