Differential Proteomics Analysis of Curcumin for Non-alcoholic Fatty Liver Disease in Rats

Objective: On the basis of proteomics,this paper researches the mechanism of curcumin in rats with non-alcoholic fatty liver disease(NAFLD) induced by high fat diet.Methods: Thirty male Wistar rats were randomly divided to three groups: normal group,model group and curcumin group.Rats in the model group and curcumin group were fed with high fat diet for 12 weeks to induce NAFLD,and simultaneously administered with curcumin in curcumin group,and with equal amount of cosolvent in model group.At the end of 12 weeks,all rats were sacrificed and their blood and liver tissue samples were collected.The levels of serum alkaline aminotransferase(ALT),aspartate aminotransferase(AST) and total cholesterol(TC) in serum and contents of triglyceride(TG) and gamma glutamyltransferase(γ-GT) in liver tissues were detected.Total protein in liver tissues was isolated by two-dimensional gel electrophoresis(2-DE),and some differentially expressed protein spots were analyzed and identified by flight mass spectrometry.Results: Compared with the normal group,the levels of ALT,AST,TC,TG and-GT in model group were significantly increased,and were significantly decreased after intervention of curcumin.The results of PD Quest software showed that there were 30 protein spots by 2-DE,and 20 significant proteins were detected by flight mass spectrometry,including mitochondrial function relevant enzymes protein and cytoskeleton protein.Conclusion: Curcumin can reduce serum indexes in NAFLD induced by high fat diet by regulating mitochondrial function and maintaining the structure of liver cells.