The HPLC analytical method of 14 kinds of vitamin contents is determined simultaneously

The invention discloses a kind of while determining the HPLC analytical method of 14 kinds of vitamin contents, it is using octadecylsilane chemically bonded silica as filler, gradient elution is carried out by mobile phase of 0.1M sodium acetate solutions methanol, the gradient flow velocity of joint mobile phase and the change of program wavelength of UV-detector, can be achieved 14 kinds of vitamins in 60 minutes（Including 4 kinds of liposoluble vitamins and 10 kinds of water soluble vitamins）Intact separation and quantitative analysis, method specificity is strong, precision is high, stability is good, the degree of accuracy is high, and easy to operate, reagent is cheap and easy to get with consumptive material, suitable for the quality control of medicine and B B-complex product food service industry, testing cost and time can be effectively saved.