Recombinant human PTEN suppresses gastric carcinoma cell growth in vitro

Objective:To study the effect of Ad-PTEN gene in SGC-7901tumor cells.To study the inhibition and potential molecular mechanisms of adenovirus-mediated expression of Ad-PTEN gene in SGC-7901 gastric cancer cells.Methods: PTEN gene was transfected into human gastric cancer cell line SGC-7901 with a replication-incompetent adenovirus vector.Using RT-PCR to detect the mRNA expression of PTEN.Using light microscope and fluorescence microscope observe the appearances of SGC-7901 cells infected by Ad-PTEN.Using MTT essay and flow cytometry to test the cell growth and apoptotic effect.Using RT-PCR to analyze apoptosis related gene expression such as Bax,Bcl-2,p53,Survivin.Results:Ad-PTEN gene infect SGC-7901 gastric cancer cells,RT-PCR revealed that the transcription of PTEN gene and its expression can inhibit SGC-7901 gastric cancer cell growth.Apoptosis may be related Bax/Bcl-2 ratio increased,Survivin decreased,p53 elevated.Conclusion: Recombinant adenovirus expressing Ad-PTEN can inhibit the growth of SGC-7901 gastric cancer cells and induce its apoptosis.