[Inhibitory effect of iguratimod on TNFalpha production and NF-kappaB activity in LPS-stimulated rat alveolar macrophage cell line].

Aim To investigate the effect of iguratimod(T-614),a non-steroidal anti-inflammatory drug,on TNFα mRNA expression and TNFα production,and on the activity of nuclear factor-κB(NF-κB) in the rat alveolar macrophage cell line(NR8383) activated by LPS. Methods NR8383 cells were pretreated with T-614(13.4,26.7, 53.4 μmol·L~(-1)),then were stimulated with LPS.The production of TNFα in the supernatant of NR8383 was assayed by enzyme-linked immunosorbent assay(ELISA).The TNFα mRNA level was determined by a semi-quantitative PCR assay.Assessment of the NF-κB DNA binding activity was performed by an ELISA kit.Results T-614 inhibited LPS-stimulated mRNA expression and production of TNFα in a concentration-dependent manner,as well as the activity of NF-κB.The IC_(50) value of effect of T-614 on TNFα level was 26.2 μmol·L~(-1).Conclusion The inhibitory effect of T-614 on the production of TNFα in LPS-stimulated NR8383 cells may be mediated by suppression of NF-κB activity.