Method for establishing in-vitro regeneration system of Osmunda vachellii

The invention discloses a method for establishing an in-vitro regeneration system of Osmunda vachellii, which belongs to the field of agricultural biotechnology. According to the method, sporangiorus is used as explant; after surface disinfection, the explant is inoculated into a variety of mediums containing an improved Beneck basal medium; prothallus is obtained through in-vitro germination of spores in sporangium; a part of the prothallus can further undergo enrichment culture, while the other part of the prothallus can be used for induced production of juvenile sporophyte (a sapling); and the sapling is transplanted into a matrix after rooting culture, and the seedling of Osmunda vachellii is formed eventually. The method provided by the invention can realize rapid and continuous obtainment of a great number of high-quality seedlings.