Abstract A54: Changes in fatty acid profile indicate a chemo-preventive response to navy bean extract in an inflammation-associated colorectal cancer mouse model

Abstracts: AACR Special Conference: Metabolism and Cancer; June 7-10, 2015; Bellevue, WA Introduction: Consumption of dry beans and their fractions decrease colorectal neoplasia; however, the underlying molecular mechanisms are unclear. Aim of the study was to identify response indicators of dietary attenuation of colorectal tumorigenesis using metabolic profiling. Methods: The study had a 2 x 2 factorial design. After being either induced or not with azoxymethane/dextran sodium sulfate (AOM/DSS), male FVB/N mice were fed an AIN93G diet containing either 0 (Control) or 10% navy bean ethanol extract (BE) for 6 weeks. Colon tissue was collected and analyzed for colitis, aberrant cell proliferation, and tumor, whereas serum, feed, and fecal samples were analyzed for metabolite levels. Results: Dietary BE attenuated AOM/DSS-induced chronic colitis, aberrant epithelial cell proliferation, fecal blood (heme), and tumorigenesis (-40%; P=0.02) in the colon. Similar changes were observed for fecal medium-chain fatty acids (hexanoate, octonoate) and plant phenolics (vanillate) and serum markers of fatty acid oxidation (carnitine, hexanoylcarnitine), and the pentose phosphate pathway (sedophetulose-7-phosphate). Moreover, dietary BE increased fecal markers of apoptosis (ribose, uracil, pseudouridine, xanthine, hypoxanthine) and attenuated the AOM/DSS-induced decrease in serum glycerophosphocholine. Conclusions: Dietary BE may inhibit survival and proliferation of premalignant colorectal epithelial cells by promoting fatty acid oxidation and resolving inflammation. Citation Format: Thushanthi H. Perera, Matthew R, Young, Shakir M. Saud, Christopher R. Dextras, Yava L. Jones-Hall, Edward D. Karoly, Brante P. Sampey, Young S. Kim, Nancy H. Colburn, Gerd Bobe. Changes in fatty acid profile indicate a chemo-preventive response to navy bean extract in an inflammation-associated colorectal cancer mouse model. [abstract]. In: Proceedings of the AACR Special Conference: Metabolism and Cancer; Jun 7-10, 2015; Bellevue, WA. Philadelphia (PA): AACR; Mol Cancer Res 2016;14(1_Suppl):Abstract nr A54.