Specific Changes in c-fos Expression and Colocalization with DNA in Identified Neuronal Nuclei of Edible Snail Following Neurotransmitter Application

The effects of serotonin and glutamate on c-fos expression and c-Fos colocalization with DNA were immunohistochemically studied in defense behavior command neurons R-LPa2-3 in snail Helix lucorum. Simultaneous neurotransmitter application resulted in increased c-Fosimmunoreactivity and colocalization with DNA-specifi c stain Hoechst 33342 in LPa2 and RPa2 neurons with specifi c dynamics for each identifi ed cell. In the nuclei of LPa3 and RPa3 neurons, neurotransmitter application did not signifi cant change the c-Fos level. These fi ndings are indicative of specifi c spatiotemporal changes in c-fos expression and c-Fos colocalization with DNA in investigated neurons of edible snail under the infl uence of neurotransmitters.