Study on enzymatic properties of Soybean Hull Peroxidase
2010
Soybean Hull Peroxidase(SHP),isolated from soybean hull,is a kind of important oxidoreductase.We extract SHP with buffer solution to determine the enzymatic activity with guaiacol method.The results show that SHP is high-temperature resisted and acid-alkai resisted,and the optimum temperature for SHP is 35 ℃,the optimum pH is 5.2,Km values of guaiacol and peroxide are 1.53 mmol/L and 0.36 mmol/L respectively.The effects of common compounds on SHP activity is totally different,for instance,Fe3+ strongly inhibition influence on SHP activity,while Mg2+ can improve the activity of SHP.
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