지구성운동과 Amino acid 병행처치가 위축된 흰쥐 골격근의 재생 및 단백질 합성에 미치는 영향

Purpose : The purpose of this study is to investigate endurance exercise and amino acid supplement induced changes in the expression of Akt, mTOR, myoD, and myogenin in the atrophied rat skeletal muscle by hindlimb suspension. Method : Male Sprague-Dawley rats(n=42) were randomly placed in one of four groups(control, supplement, exercise, supplement&exercise) after 10days atrophy by hindlimb suspension. The Rat in the exercised groups ran the treadmill running for the intensity 30~60min 8~24M/min with degree slope 0°. The Rat in the supplemented groups had oral supplementation of 1ml of Amino acid twice per day(am/pm). The western blotting analysis performed for the expression of Akt, mTOR, myoD, myogenin protein. Results : The expression of Akt significantly increased in one week and gradually decreased in following second and fourth weeks. The expression of mTOR significantly increased in the second week than the first week but decreased in the fourth week. The expression of myoD significantly increased gradually by time passing. The expression of myogenin increased in one week and gradually decreased in following weeks and increased insignificantly in supplemented group. Conclusion : This study can be concluded that combinational treatment with endurance exercise and amino acid supplement has positive effects on recovery of atrophied skeletal muscle by controlling expression of Akt, mTOR, myoD and myogenin involved in protein synthesis and muscle regeneration.