«Изучение биосовместимости интракорнеальных линз в экспериментально-морфологическом исследовании ex vivo»

Currently, several types of intracorneal lenses (ICL) are used in clinical practice to correct presbyopia [5]. It seems relevant to search for biocompatible material that is inert with respect to corneal stromal tissue, in the presence of which corneal stromal cells have a reduced tendency to adhesion and proliferation [7‒10]. In this study, 2 different polymers based on hydroxyethylmethacrylate (HEMA) and olygouretanmethacrylate (OUMA) were selected. [11‒14].The aim is to study the reaction of the cadaver corneas to the implantation of ICL made of polymer materials based on HEMA and OUMA in an experimental study ex vivo.Methodology. To model the process of intracorneal implantation, we used the eyeballs of cadaver donors (EBCD). Four experimental groups were formed: 1. The experimental group of "HEMA" (3 EBCD). 2. An experimental group of "OUMA" (3 EBCD). 3. The comparison group of "Chronic control" (4 EBCD). 4. The comparison group of "Acute control" (3 EBCD). At the end of the study, the presence of fibrous connective tissue elements on the studied surfaces and the degree of ICL deformation were evaluated. Qualitatively compared the images in the experimental and control groups, as well as obtained by various methods – fluorescence microscopy and scanning electron microscopy.Results. According to the results of the ex vivo study, the developed lenses did not tend to encapsulate. On the surface of the ICL in the experimental groups cellular-fibrous elements were found, however, there was no monolayer of cells or fibrous elements on thee ICL in experimental groups, and therefore it can be assumed that the studied polymer materials have minimal adhesive properties.Conclusions. Thus, the results of the experimental study ex vivo show that ICLs made of both polymer materials (HEMA and OUMA) are potentially suitable for intracorneal implantation and can be recommended for further study in clinical conditions, because of its biologically compatible.