Furosemide Determination by First‐Derivative Spectrophotometric Method

Abstract Furosemide was analyzed in tablet and liquid pharmaceutical preparations entirely and after photolitic degradation, by direct ultraviolet first derivative spectrophotometry 1 D at 262 nm. At this wavelength, it was possible to eliminate the decomposition product interference. A linear response range between 1.0 and 10.0 µg mL−1 was obtained in optimized conditions. The estimation of relative standard deviation was within the range 0.37–2.4% (n=10). The method was applied in pharmaceutical preparation samples, and the results were compared with those obtained with normal spectrophotometric method to determine furosemide with advantages as high selective, accuracy and precision