Reliability of biotinylated DNA probes in colony hybridization: evaluation of an improved colony lysis method for detection of TEM-1 β-lactamase

1988 
Abstract Utilizing an improved method for colony hybridization developed by Haas & Fleming, biotin and 32 P-labelled TEM-1 probes were compared for sensitivity and specificity in identifying the type of β-lactamase made by over 100 clinical bacterial isolates. The new procedure was more reliable than a standard one, but still gave more than 20% false positive and false negative reactions.
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