Effects of myxoma virus on gliomas of rats models in vivo

Objective To explore the in vivo effects of myxoma virus (MV) on gliomas of rat model.Methods C6 glioma cells were implanted into the frontal lobe of SD rats using stereotactic methods to establish animal models of glioma.Methods C6 glioma cells were implanted into the frontal lobe of SD rats using stereotactic methods to establish animal models of glioma.Models were divided into 4 groups randomly after tumor growth was affirmed,and MV,5-FU,MV + 5-FU,and denatured myxoma virus ( DV ) were implanted into the tumors using stereotactic methods,bodyweight,tumor size,expression of glial fibrillary acidic protein ( GFAP),Akt of each model were observed.Results The gliomas in all SD rats were established successfully. And tumor growth in MV,5-FU,MV + 5-FU were significantly decreased as compared with DV group after injection,sizes of some tumors were lessened,and GFAP expression decreased in MV,5-FU and MY + 5-FU groups.The expression of PI3k,Akt and mTOR were decreased in MV and MV + 5-FU groups.Conclusion C6 glioma SD rat models could be established successfully using stereotactic methods.MV may enhance biological activity of chemotherapeutic drugs on tumor cells of animal models in vivo by regulating some genes of PI3K-Akt-mTOR signal pathway. Key words: Glioma; Myxoma virus; Models,animal; in vivo