Investigation of Antioxidant Enzymes of Amsonia orientalis in the Presence of Salt Stress and BAP / Tuz Stresi ve BAP Varlığında Amsonia orientalis’in Antioksidan Enzimlerinin İncelenmesi

Amsonia orientalis Decne (Apocynaceae), is a threatened plant species providing many glycosides and glycoalkaloids which have medicinal and economic significance. The species has a natural distribution only in the north-west of Turkey and the north-east of Greece. Abiotic stress factors are tought to be  major causes of plant limited distribution. . Thus, the effect of the increasing salt concentrations onMDA and H 2 O 2 content and antioxidant enzymes (SOD, CAT, POD) of Amsonia orientalis was investigated in the presence of 1.0 mg/mL benzylaminopurine (BAP)-due to sooth effect of BAP- spectrophotometrically and electroprotometrically. It was found that salt stres decreased to H 2 O 2 contend but elevated at high concentrations of NaCl and  enhanced the specific activities of antioxidant enzymes tested. Electrophotometric analysis showed that Fe-SOD and Cu/Zn-SODs activities were inhibited while Mn-SOD, CAT and POD activities remained their activities against increasing salt stres. Amsonia orientalis Decne. (Apocynaceae), dunyada sadece Turkiye’nin kuzeybatisi ve Yunanistan’in kuzeydogusunda yayilis gosteren, tibbi ve ekonomik oneme sahip yapisinda cok sayida glikozitler ve glikoalkaloitler bulunduran, nadir ve tukenme tehdidi altinda olan bir bitki turudur. Bitkinin tukenme tehlikesi altina girisinin onemli sebeplerinden biri olarak giderek artan abiyotik stres kosullari dusunulmektedir. Calismamizda, tuzluluk stresinin Amsonia orientalis’in H 2 O 2 ve MDA seviyeleri ile antioksidan enzimlerinin (SOD, CAT, POD) uzerindeki etkisi; stresi yatistirmak amaciyla 1,0 mg/mL benzilaminopurin (BAP) varliginda elektrofotometrik ve fotometrik olarak irdelelenmistir. Artan tuz stresi kosullarinda H 2 O 2 ve MDA seviyelerinin benzer olarak yuksek tuz konsantrasyonlarinda arttigi ancak dusuk tuz konsantrasyonlarinda azaldigi; uc antioksidan enzimin de spesifik aktivitelerinin arttigi gozlenmistir. Elektrofotometrik analizler sonucunda artan tuz stresinin bitkinin Fe-SOD ve Cu/Zn-SOD aktivitelerini inhibe ettigi, Mn-SOD aktivitesinde degisiklik olusturmadigi, CAT ve POD aktivitelerinin ise tum tuz konsantrasyonlarinda korundugu gozlenmistir.