Production of chymosin and chymosin precursor with microorganism
1992
PURPOSE: To produce a substitute for rennet necessary for the production of cheeses by using a microorganism containing a recombinant DNA. CONSTITUTION: This method for producing a chymosin or its precursor comprises transforming a microorganism with a recombinant plasmid containing a proper regulon and a specific structure gene encoding for bovine calf preprochymosin having a specific base sequence or one of various allelic or maturation forms thereof, culturing the transformant and subsequently isolating the preprochymosin or one of the various allelic or maturation forms thereof. COPYRIGHT: (C)1993,JPO
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