Determinationof Single HybridizationTemperaturefor Serotyping of HumanGroupA Rotaviruswith Oligonucleotide Probes

The possibilityof use of a singlehybridization temperatureinsteadof different temperaturefor differentprobes for detectionof humangroup A rotavirus(HRV)was ex- plored. The sensitivityand specificityof the 5 oligonucleotide probes were assessed using cell culture adapted rotavirus (RV) strains by carrying out hybridization at a fixed temperatureof 50Ž. The results obtainedindicatethat a single hybridizationtemperature may be used for all the probes with virtuallyno decreasein specificityand sensitivity.