Use of flow and laser scanning cytometry to study mechanisms regulating cell cycle and controlling cell death

This article presents major applications of flow and laser scanning cytometry in studies of cell cycle and cell neurobiology. Described are representative examples of multivariate analysis of different attributes of the cell that reveal correlations between cellular DNA content; DNA replication; and expression of cyclins D1-3, E, A, and B1. Also described are major applications of cytometry in studies of apoptosis, specifically in measurement of Bax translocation to mitochondria and nuclear factor kappa B to the nucleus, and in detection of DNA fragmentation.