An integrated genetic and man-mouse comparative map of the DXHXS674-Pdha1 region of the mouse X chromosome

Abstract The genes for ocular albinisim type 1 (OA1) and the Xenopus laevis -like apical protein (APXL) map between amelogenin (AMELX) and the pseudoautosomal boundary in the distal region of the human X chromosome short arm. The mouse homologues, Oa1 and Apxl, have recently been shown to lie proximal to their expected locations on the mouse X chromosome, but their positions with respect to critical gene loci in the vicinity have not been defined. By analyzing recombination events from ( Mus musculus × Mus spretus ) × M. musculus backcrosses, we have constructed a detailed mouse genetic map that encompasses Oa1, five other genes, and 13 microsatellite loci. The order of genes and evolutionary breakpoints (EB) is defined as centromere–(EB)–( DXHXS674, DXHXS679 )– Smcx –(EB)– Oa1 –(EB)– Phex (3′ → 5′)– Pdha1 –telomere. Thus Oa1 lies in a region between two previously characterized conserved segments.