Additional file 1: Figure S1. of Novel cationic peptide TP359 down-regulates the expression of outer membrane biogenesis genes in Pseudomonas aeruginosa: a potential TP359 anti-microbial mechanism

Differential gene expression levels of selected genes for the mucoid and non-mucoid strains of P. aeruginosa exposed to TP359 bactericidal treatment. P. aeruginosa at 1 × 109 CFU/mL were either untreated as control, or treated with 4× the MIC of TP359 and then grown for 10 and 30 min. Total RNA was extracted, quantified before cDNA synthesis using reverse transcriptase, followed by PCR amplification. Data obtained from three-independent experiments were used to analyze the relative gene expression by the 2-ΔΔCt method. Panels A and B show the 10 and 30 min treatment for the mucoid strain and non-mucoid strain, respectively. (JPG 145 kb)