Prostaglandin E1 Reduces Compression Trauma–Induced Spinal Cord Injury in Rats Mainly by Inhibiting Neutrophil Activation

Prostaglandin E1 (PGE1), a potent vasodilator, was recently reported to inhibit both neutrophil activation and monocytic production of tumor necrosis factor-α (TNF-α) in vitro. We previously reported that TNF-α was critically involved in the development of motor disturbances by increasing the accumulation of neutrophils at the site of injury in rats subjected to compression trauma-induced spinal cord injury. Therefore, it is possible that PGE1 reduces motor disturbances by inhibiting neutrophil activation in rats subjected to spinal cord injury. We examined this possibility in a rat model of spinal cord injury (SCI). Motor disturbances induced by spinal cord compression were evaluated using the inclined plane test, and footprint analysis. Accumulation of neutrophils at the site of trauma was evaluated by measuring tissue myeloperoxydase (MPO) activity. Tissue levels of TNF-α were determined using an enzyme-linked immunosorbent assay. Motor disturbances induced by spinal cord compression were significantly...