Determination of Selected Colored Smokes on Glass Fiber Discs by High Performance Liquid Chromatography (HPLC)

Abstract : Liquid chromatographic method has been developed for the determination of 1,4-diamino-2,3-dihydroanthraquinone (DDA), 2-(21-quinolinyl)- 1,3-indandione (QID), 1-methylamino-anthraquinone (MAA), and 1,4-di-p- toluidinoanthraquinone (DTA). A relatively rapid and reliable HPLC method has been developed for the determination of selected colored smokes on glass fiber filter discs. The sample preparation process was streamlined by extracting the colored-smokes/disc samples into acetonitrile followed by filtration and direct injection into a high performance liquid chromatograph equipped with a programmable ultraviolet (UV) spectrophotometric detector. A mixed-mode C18/ cation column provided adequate resolution and optimal peak symmetry for all four of the colored smokes investigated. The precision and accuracy data and detection limits are included in the report.