Evaluation of paraoxonase, arylesterase, and homocysteine thiolactonase activities in patients with diabetes and incipient diabetes nephropathy

Uvod: Cilj ovog istraživanja je da se ispita odnos između promena u aktivnostima paraoksonaze (PON), arilesteraze (ARE) i homocistein tiolaktonaze (HTLase) sa antioksidativnim svojstvima i razvojem dijabeticke nefropatije (DN), jednog od najcescih komplikacija dijabetesa. Methods: Normoalbuminuric type-2 diabetic patients (Group II, n=100), microalbuminuric type 2 diabetic patients (Group III, n=100) and the control group (Group I, n=100) were included in the study. The age and gender of the patient groups matched with the age and gender of the control group. HTLase, PON and ARE enzyme activities were measured by the spectrophotometric method using a g-thiobutyrinolactone, paraoxon, and phenylacetate substrates respectively. In this study, an autoanalyzer application was developed in order to measure HTLase enzyme activity for the first time. Rezultati: Aktivnosti serumske HTLaze, ARE i PON kod grupe III i grupe II bile su znacajno niske u poređenju sa rezultatima HTLaze, ARE i PON kod grupe I (p < 0,05). Zakljucak: Na osnovu nasih rezultata utvrđeno je da su aktivnosti enzima PON, ARE i HTLase smanjene zbog povecanja stepena DN.