Studies on chemical constituents of Ranunculus ternatus

Objective:To study the active constituents of Ranunculus ternatus.Method:The constituents were isolated with silica gel and Sephadex LH-20 gel column chromatography and purified by HPLC.Their structures were elucidated by spectroscopy.Result:Sixteen compounds were obtained and identified as Stigmasta-4,6,8(14),22-tetraen-3-one(1),5-hydroxymethyl furaldehyde(2),γ-keto-δ-valerolactone(3),pantolactone(4),5-hydroxymethyl-dihydro-furan-2-one(5),methyl 5-hydroxy-4-oxopentanoate(6),methyl hydrogen succinate(7),succinic acid monoethyl ester(8),3,4-dihydroxybenzoic acid methyl ester(9),p-hydroxycinnamic acid(10),4-oxo-pentanoic acid(11),succinic acid(12),nonanedioic acid(13),4-hydroxybenzoic acid(14),4-hydroxybenzaldehyde(15),stigmasterol-3-O-β-D-glucopyranoside(16).Conclusion:Compound 1,4-15 are obtained from this plant for the first time.