Incorporation of bacteria in concrete: The case against MICP as a means for strength improvement

Abstract Strength improvement of cement-based materials by the addition of bacteria has been reported over the past decade and has been mainly attributed to microbially induced calcite precipitation (MICP 1 ). However, the ability of bacteria to survive, grow and retain their metabolic activity in concrete is questionable. This research aims to shed light on the mechanisms involved in the strength enhancement of cementitious materials that contain bacteria. The addition of different concentrations of live and dead cells of Bacillus cohnii in cement mortars led to an increase in flexural and compressive strength for the mortars containing both types of bacteria. Findings of the present study led to exclusion of MICP as the main cause of strength enhancement, disproving earlier theories. Other known hypotheses including the behaviour of bacteria as organic fibres or as nucleation sites are thoroughly discussed, and a new approach is proposed.