Enzyme-linked immunosorbent assay for the detection of infectious bronchitis virus antiges.

Enzyme-linked immunosorbent assay (ELISA) to detection of infectious bronchitis virus (IBV) antigen was applied. IBV M41, A-5968 and L2 strains showing strain specificity in virus neutralizing test showed marked cross-reactivity. IBV M41 antigen was detectable from both SPF eggs and chickens inoculated with the virus. Between the positive (purified IBV and infectious allantoic fluid) and negative controls (Newcastle disease virus B1 strain and allantoic fluid from SPF eggs), there were marked differences in ELISA titers. From these results, ELISA was considered to be a speedy and good technic for detecting IBV antigens from test samples infected with the virus experimentally.