Demographics, tumor characteristics, and clinical outcomes associated with somatic mutations in 201 cancer-related genes in advanced melanoma patients (pts).

9057 Background: Cutaneous melanomas (CM) have a high rate of somatic mutations. We analyzed the prevalence and correlations of mutations in 201 cancer-related genes to identify clinically significant molecular events in a cohort of pts with advanced melanoma. Methods: Next generation sequencing (NGS) of all exons of 201 cancer-associated genes was performed on tumor and germline DNA from pts with a known primary non-acral CM enrolled in an IRB-approved research protocol. Demographics, primary tumor characteristics, date of diagnosis of stage IV disease, and overall survival (OS) were collected for all pts. The significance of associations between these characteristics and somatic mutations was analyzed by a variety of statistical methods. Results: NGS was performed on 106 advanced melanoma pts with a known non-acral primary CM. Genes with a mutation rate > 10% (n = 74) were assessed for clinical associations. NF1 (OR 12.9, p = 0.002) and PCLO (OR 3.1, p = 0.01) mutations were associated with male gender,...