Chemometric Assisted New Stability Indicating NP-HPLC Method Development and Validation of Doxycycline and Lactobacillus in Combined Dosage Form -

A new chemometric assisted by high-performance liquid chromatography (HPLC) with photodiode array (PDA) detection was implemented for the simultaneous determination of Doxycycline and Lactobacillus in capsule dosage form. Two chemometric calibration techniques, principle component analysis (PCA) and partial least squares (PLS) were applied to the peak area at 225 nm of PDA detector responses. The method was carried out on chiral cell OJ-RH (150 X4.6mm, 3.5um) a column with a mobile phase consisting of Hexane and Ethyl acetae in the ratio of (90:10%v/v) and flow rate of 1.0 ml/ min. The detection was carried out at 225nm.The retention time for Doxycycline and Lactobacillus were found to be 3.013and 7.267 min respectively. The method was validated according to the ICH guidelines for specificity, LOD, LOQ, precision, accuracy, linearity and robustness. The method showed good reproducibility and recovery with %RSD less than 2. So the proposed method was found to be simple, specific, precise, accurate and linear. The ‘UNSCRAMBLER (camo)’software was used for the numerical calculations. All of the two-chemometric analysis methods in this study can be satisfactorily applied for the quantitative analysis of Doxycycline and lactobacillus pharmaceutical capsule dosage form