A Transcript Map of a 2-Mb BAC Contig in the Proximal Portion of the Mouse X Chromosome and Regional Mapping of the scurfy Mutation

Abstract A physical clone contig has been constructed, spanning 2 Mb on the proximal mouse X chromosome containing the mouse scurfy ( sf ) and tattered ( Td ) mutations. Extensive transcript mapping in this interval has identified 37 potential transcription units, including a number of novel genes, and 4 pseudogenes. These genes have been ordered by STS content and restriction mapping. Comparison of the transcript map to the corresponding region in human Xp11.23–p11.22 shows extensive homology, with complete conservation of gene order for loci in common between the two maps. Further, using a novel method to identify simple sequence length polymorphisms, we have developed a number of genetic markers, which has enabled the region containing the sf mutation to be narrowed to Td gene using a candidate gene approach and now serves as a starting point for the cloning of the sf mutation.