Development and Validation of Two Solid-Phase Enzyme Immunoassays (ELISA) for Quantitation of Human Epidermal Growth Factors (hEGFs)

Nywana Sizemore,Richard C. Dudeck,Charles M. Barksdale,Gerald D. Nordblom,Williams T. Mueller,Patrick I. McConnell,D. Scott Wright,Antonia Guglietta,Be-Sheng Kuo

Development and Validation of Two Solid-Phase Enzyme Immunoassays (ELISA) for Quantitation of Human Epidermal Growth Factors (hEGFs)

1996

Nywana Sizemore
Richard C. Dudeck
Charles M. Barksdale
Gerald D. Nordblom
Williams T. Mueller
Patrick I. McConnell
D. Scott Wright
Antonia Guglietta
Be-Sheng Kuo

Purpose. The purpose of the present investigation was to develop and validate two separate enzyme-linked immunosorbent assays (ELISA) for quantitation of exogenous human epidermal growth factor (hEGFl -53) and its truncated fragment (hEGFl-48) in rat plasma.

Keywords:

Epidermal growth factor
Enzyme immunoassays
Quantitative analysis (chemistry)
Biochemistry
Human epidermal growth factor
Chemistry
Molecular biology
pharmacology toxicology
method development
elisa assay
Chromatography

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