高羊茅甘油醛－3-磷酸脱氢酶基因FaGAPDH的克隆与分析

Taking the stem base and the leaf base of tall fescue as temples, with primers designed based on the sequence of GAPDH gene from Lolium perenne L., two full-length cDNAs of GAPDH genes were cloned from tall fescue by RT-PCR combined with 3'RACE and 5'RACE. The two newly cloned GAPDH genes were designated as FaGAPDH1 (GQ480772) and FaGAPDH2 (GQ480773). Sequence analysis shows that FaGAPDH1 is 1 281bp in length, and contains a single open reading frame from 74bp to 1 087bp. FaGAPDH2 is 1 348bp in length, and contains a single open reading frame from 106bp to 1 119bp. They both encode proteins with 337 amino acids. Domain analysis reveals that proteins encoded by the two genes both contain two conserved domains: NAD (P)-binding Rossmann-fold domains and C-terminal domain. Similarity comparison between FaGAPDH1 protein, FaGAPDH2 protein and the protein encoded by GAPDH gene from gramineous plants shows that they own amino acids sequence similarities of more than 90%, indicating that the two new genes are both highly conservative.