Application of gene array chip in diagnosis of cytogenetics of recurrent spontaneous abortion

Objective To investigate clinical value of single nucleotide polymorphism array (SNP-array) gene chip technique in diagnosis of genetics of recurrent spontaneous abortion (RSA).Methods From January to October 2012,the 26 patients with more than twice of spontaneous abortion in Huzhou Maternal and Child Health Care Hospital were enrolled in this study (RSA group).Meanwhile 20 cases with induced abortion were taken as control group.All aborted tissues were analyzed with conventional cytogenetic karyotyping and SNP-array,respectively. Results Chorionic villus chromosomal examination was successfully done in 19 cases (73%,19/26),which 10 cases were found with chromosomal anomaly,the overall detection rate is 10/19.However,SNP-array analysis was successfully performed in all 26 cases.The overall rate of detection was 100%,and abnormalities were found in 15 cases,which reached the detection rate was 58% (15/26).Chorionic villus chromosomal examination was successfully done in 16 cases (16/20) in control group,and none of the resluts was chomosomal anomaly;SNP-array analysis was successfully performed in all 20 cases (20/ 20),and none was found abnormalities. Conclusion SNP-array gene chip technique showed highly accurate feature,which could be used in cytogenetic diagnosis of recurrent spontaneous abortion. Key words: Abortion,habitual; Oligonucleotide array sequence analysis; Polymorphism,single nucleotide; Chorionic villi; Karyotyping