The role of bridge sequence in the construction of artificial ribozyme M1GS

Aim: To elucidate the influence of covalently GS on the conformation and function of M1RNA in M1GS. Methods: Applying the RNA second structure software to simulate structure of M1GS, a sequence folded independently as bridge between M1RNA and GS was screen out. The enzyme activities of M1GS with bridge and M1GS without bridge were tested in vitro. Results: The M1GS with bridge can efficiently cleave the substrate, UL54 RNA fragment, however, the M1GS without bridge don't show activity of cleavage. Conclusion: It confirm that the bridge contribute to the completeness of confirmation of M1RNA, and play an important role in keeping the M1GS' activity of in vitro cleavage.