Additional file 4: Figure S4. of IMPACT is a GCN2 inhibitor that limits lifespan in Caenorhabditis elegans

Impt-1 knockdown promotes stress resistance. (a, b) Lifespan assays under heat stress (28 °C) of N2 worms and impt-1 +/– (ok3233) mutants (a) or N2 worms treated with L4440 (control) or impt-1 RNAi from L1 (b). Values of median lifespan and statistics are reported in Additional file 10: Table S1. Survival curves were compared by log-rank test. Experiment (a) was repeated three times and (b) was performed once. Data demonstrate the composite of experiments XXXII–XXXIV (a) or the experiment XXXV (b). (c) Fraction of dead N2, impt-1 +/– (ok3233) and gcn-2(ok871) worms when exposed to 5 mM or 7.5 mM sodium arsenite for 7 hours (N2, n = 44; impt-1 +/– , n = 46; gcn-2, n = 48; *P