Tissue rapid reproduction technology of self-incompatible line in headed cabbage.

The factors of explants,hormone combination and concentration and plant mode were investigated on tissue rapid reproduction technology of self-incompatible line in headed cabbage.The results indicated that the best medium to induce callus was MS+6-BA 5 mg/L+NAA 0.25 mg/L and regeneration rate were 87.5% and 85%,respectively.The media of 2 mg/L 6-BA+0.01 mg/L NAA and 3 mg/L 6-BA+0.1 mg/L NAA were the best for inducing adventitious buds.The plantlet in vitro multiplication coefficience was more than 4.It is easy for inducing adventitious roots on 2/3MS medium supplement with 0.1 mg/L IBA and 0.1 mg/L NAA and the average number roots were 7～8 and rooted rate was 80.3%.The survive rate of plantlet in vitro that was planted in cultivating plug was 100% contrary to 84.7% survived in field directly.The experiments also showed that axillary buds propagated faster than that of the hypocotyl in the beginning of the culture,but there were not significant difference between them on successive transfer culture,multiplication,rooted and growth in field.