What is the best laboratory method for diagnosis of Herpes Simplex Virus in genital infections

Genital infection caused by Herpes simplex virus (HSV) is one of the most common health problems, worldwide. Several methods such as cell culture, serological and molecular methods have been used to detect this virus. Currently, Real-Time Polymerase Chain Reaction (Real time-PCR) technique is widely used due to its high sensitivity and specificity. Besides, Real time-PCR can be employed in the follow-up of therapeutic effects in HSV-infected person who is being treated with antiretroviral drugs. We conducted a review on traditional and current diagnostic methods with a focus on their limitations in the diagnosis of HSV infection.