A FIBERSCOPE FOR SPATIALLY SELECTIVE PHOTOACTIVATION AND FUNCTIONAL FLUORESCENCE IMAGING IN FREELY BEHAVING MICE

Cathie Ventalon,Vivien Szabo,Vincent de Sars,Jonathan Bradley,Valentina Emiliani

A FIBERSCOPE FOR SPATIALLY SELECTIVE PHOTOACTIVATION AND FUNCTIONAL FLUORESCENCE IMAGING IN FREELY BEHAVING MICE

2015

Cathie Ventalon
Vivien Szabo
Vincent de Sars
Jonathan Bradley
Valentina Emiliani

We demonstrate targeted photoactivation with near-cellular resolution and fluorescence imaging with optical sectioning in freely-behaving mice. Photoactivation patterns were produced with computer-generated holography and transmitted to the mouse using a fiber bundle.

Keywords:

Neuroscience
Holography
Fiberscope
Fluorescence microscope
Fluorescence-lifetime imaging microscopy
Functional imaging
Optical sectioning
Analytical chemistry
Medicine
Biomedical engineering
a fibers
computer holography

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