Liquid chromatographic process identification using pulse testing techniques applications to column standardization and scale-up

Transfer functions have been obtained from frequency response analysis via liquid chromatographic pulse testing and Bode diagrams. The system order of the transfer function, within the range tested, is dependent on the anion-exchange gel characteristics, and not on packing height or pulse sample size. It is also observed that as the activity of the anion-exchange gel decreased, the system order of the transfer function decreased. This unique identification strategy could have great potential for the design of automatic control systems, and the standardization of chromatographic systems for Food and Drug Administration process validation. The ultimate goal, however, would be to verify phenomenological models to facilitate process scale-up for the commercialization of bioproducts.