Individual Lysine Acetylations on the N Terminus of Saccharomyces cerevisiaeH2A.Z Are Highly but Not

, -K10 , and -K14 showthat all three modifications are added by Esa1 acetyltrans-ferase and removed by Hda1 deacetylase. Completely unacety-latablehtz1alleles exhibit widespread interactions in genomescale genetic screening. However, singly mutated (e.g. htz1-K8R) or singly acetylable (e.g.the triple mutanthtz1-K3R/K10R/K14R) alleles show no significant defects in these analy-ses. This suggests that the N-terminal acetylations on Htz1 areinternally redundant. Further supporting this proposal, eachacetylation decays with similar kinetics when Htz1 transcrip-tion is repressed, and proteomic screening did not find a singlecondition in which one Htz1