Rapid detection of different dna analytes using a single electrochemical sensor

Abstract A unique platform for the detection of two distinct DNA sequences by using one single electrochemical sensor was developed. The sensor consists of a universal stem loop probe attached to a solid surface, and two analyte-specific adaptor strands. These adaptor strands hybridize to a nucleic acid analyte and provide both highly specific recognition and high binding affinity. The universal probe can be regenerated by a simple and quick rinse with urea and water. As a proof of concept, we differentiated long target sequences of Zika (141 nt.) and Dengue (114 nt.) viruses that contain secondary structures. The proposed sensor provides a platform for rapid and cost-efficient detection of potentially any DNA or RNA sequence using a single electrochemical sensor.