Identification of DNA sequences involved in host specificity in the pathogenesis of Pseudomonas solanacearum strain T2005

Two genomic libraries were constructed in Escherichia coli from DNA of Pseudomonas solanacearum wild-type strains T2003 (pathogenic to potato, but nonpathogenic to peanut) and T2005 (pathogenic to peanut) by using a broad host range vector pLAFR1. Transferring clones from the strain T2003 library into a strain T2005 derivative recipient, followed by plant pathogenicity tests, revealed no presumptive avirulence gene in the nonpathogenic P. solanacearum donor strain. (…)